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While it is crucial to note that not all individuals with autism engage in substance abuse, studies have shown higher rates of alcoholism, drug addiction, gambling addiction, and internet addiction among individuals on the autism spectrum

Cells were lysed using mild lysis buffer [20 mM tris at pH 7.5, 150 mM NaCl, 5 mM EDTA, 1% NP-40, 10% glycerol, 1 protease inhibitor cocktail, and 1 phosphatase inhibitor (Roche)]

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They can perform physical examinations and, if necessary, order diagnostic tests to identify the underlying cause