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Briefly, the cauda epididymis was dissected from each mouse and placed in a pre-warmed M2 medium (Sigma, M7167) before gently squeezing the caudal tissue to release sperm into the media

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Tobacco use Cigarette smoking Approximately one in every nine participants (11.7%, n = 665) in this study reported smoking, and more than half (57.8%, n = 366) of mothers who smoked pre-pregnancy continued to smoke after having their baby

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