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Repeat steps 13.1 through 13.4 with the second and third cigarette

Interestingly, addition of PTIO, a scavenger of nitric oxide (NO) showed slight but significant inhibition of the cell death suggesting the role for combustion-mediated generation of NO in smoke-dependent cell death induction in plant cells (Figure 5)

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1 Babies whose mothers smoke while pregnant or who are exposed to secondhand smoke after birth have weaker lungs than other babies, which increases the risk for many health problems