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The annealed primer mix was then ligated to the BstZ17I/NgoMIV digested and CIP treated plasmid according to the New England Biolabs (NEB

Electronic cigarettes (E-Cigs) and nicotine replacement therapy (NRT) Although the prevalence of tobacco use has declined, other methods of nicotine consumption, such as electronic nicotine delivery systems and nicotine replacement therapies (NRTs), have become increasingly popular [168]

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